Fatty Acid Acylated Proteins of the Halotolerant Alga Dunaliella salina
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چکیده
منابع مشابه
Fatty Acid Acylated Proteins of the Halotolerant Alga Dunaliella salina.
The unicellular, wall-less alga Dunaliella salina has been shown to contain an array of proteins modified by the covalent attachment of fatty acids. Myristic acid (14:0) comprised approximately 80% by weight of the protein-linked acyl groups in samples derived from cells cultured in medium containing 1.7 molar NaCl and 93% in samples from cells grown in medium containing 3.0 molar NaCl. Palmiti...
متن کاملIdentification of Low Molecular Mass GTP-Binding Proteins in Membranes of the Halotolerant Alga Dunaliella salina.
A family of specific guanine nucleotide-binding proteins in Dunaliella salina was studied. Polypeptides of different subcellular fractions were separated by electrophoresis and transferred to nitrocellulose or Immobilon membranes. Incubation of the transfer blots with [(35)S]GTPgammaS or [alpha-(32)P]GTP showed no evidence for GTP-binding proteins in the chloroplast and cytosol fractions. Howev...
متن کاملHalotolerant Alga Dunaliella safina'
Na+/H+ exchange activity in whole cells of the halotolerant alga DunaIfeila saUna can be elicited by intracellular acidification due to addition of weak acids at appropriate external pH. The changes in both intracellular pH and Nat were followed. Following a mild intracellular acidification, intracellular Nat content increased dramatically and then decreased. We interpret the phase of Nat influ...
متن کاملSalt induction of fatty acid elongase and membrane lipid modifications in the extreme halotolerant alga Dunaliella salina.
In studies of the outstanding salt tolerance of the unicellular green alga Dunaliella salina, we isolated a cDNA for a salt-inducible mRNA encoding a protein homologous to plant beta-ketoacyl-coenzyme A (CoA) synthases (Kcs). These microsomal enzymes catalyze the condensation of malonyl-CoA with acyl-CoA, the first and rate-limiting step in fatty acid elongation. Kcs activity, localized to a D....
متن کاملNa-NMR Studies of the Intracellular Sodium Ion Concentration in the Halotolerant Alga Dunaliella salina.
The Intracellular Na(+) concentration in the halotolerant alga Dunaliella salina was measured in intact cells by (23)Na-NMR spectroscopy, utilizing the dysprosium tripolyphosphate complex as a sodium shift reagent, and was found to be 88 +/- 28 millimolar. Intracellular sodium ion content and intracellular volume were the same, within the experimental error, in cells adapted to grow in media co...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1989
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.90.2.549